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Salmonella PCR Fluorescence Quantitative Diagnostic Kit,Gaiton Technology,Inc.
 
 
 
Salmonella PCR Fluorescence Quantitative Diagnostic Kit
 
Release Date:2009-3-25 17:10:35  Views:1111  Category:PCR kit
 

Introduction

Salmonella is a type of bacteria. It is usually found in poultry, eggs, unprocessed milk and in meat and water. It may also be carried by pets like turtles and birds. Salmonella infections may cause excessive diarrhoea, stomach cramps and general health problems.

This kit is a PCR fluorescence assay for qualitative detection of Salmonella (also known as highly pathogenic avian influenza) in vitro. It is intended for identification of infection with the Salmonella viruses.

 

Principle

This kit contains a specific Salmonella primer. During the PCR reaction, the DNA polymerase cleaves the probe and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially is proportional to the amount of the specific PCR product.

 

Kit Contents

Type of reagent(48T/kit)

Type

vial 

DNA Extraction Buffer

3ml   

1 vial

Salmonella Reaction Mix

1100ul

1 vial

Taq Enzyme(5U/ul)

20ul  

1 vial

Salmonella Positive Control

100ul 

1 vial

Internal control

100ul 

1 vial

 

Sensitivity: 100 copies /ml

 

Liner range:10-1010 copies /ml

 

DevicesABI7500,9600,MJ opticon2,Bio-Rad

 

48 tests /kit

 

 
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